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Swab:Direct™ Extraction Kit

Dramatically reduce extraction time.

FEATURES

Fast and simple method to extract DNA – Whole process in 5 minutes.

Minimized hands-on time.

Ideal for on-site testing.

Room temperature storage.

01 Swab:Direct™ Extraction Kit

  • Swab:Direct™ Extraction Kit is designed to perform PCR test from nasopharyngeal and oropharyngeal swab samples without prior DNA/RNA purification process. The buffer includes high concentrated alkaline compounds for lysis and elution of sample organisms. This product is easy to use by just mixing the swab sample and extraction buffer without additional processes for preparing nucleic acid template to run PCR tests.

02 RESULTS OF PERFORMANCE VERIFICATION TESTS

  • To comparative experiments between Swab:Direct™ Extraction Kit and others, carried out using GAPDH gene extracted from sample collected by nasopharyngeal and oropharyngeal swab. Each sample was extracted five times per each method. Perform real-time PCR analysis of the extracted nucleic acid from each extraction method using GENECHECKER® UF-300 Real-time PCR System. The relatively lower Ct value was identified than the other methods, which resulted in higher efficiency.

    Swab:Direct™ Extraction Kit

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab 29.82 31.01 30.65 30.96 31.64 30.82
    2 Oropharyngeal swab 30.36 31.68 30.94 32.35 31.46 31.36

    Spin Column Method ("A" company)

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab 31.96 30.88 31.81 31.68 31.53 31.57
    2 Oropharyngeal swab 31.45 31.97 32.16 31.25 32.35 31.84

    Magnetic Bead Method ("B" company)

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab 35.15 34.76 35.93 36.57 35.06 35.49
    2 Oropharyngeal swab 35.33 35.49 35.27 34.60 34.74 35.09

PROTOCOL

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KIT CONTENTS

Item Quantity / Volume Description
Swab:Direct™ Extraction Kit 300 μL x 100 tubes Extraction Buffer

ORDERING INFORMATION

Cat. No.
Description
Pack Size
9799140105
Swab:Direct™ Extraction Kit
100 tubes/PK

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Rapi:Direct™ Lysis Buffer

Makes 'Direct' PCR possible.

FEATURES

Fast and simple method for DNA extraction – whole process in 10 minutes

Minimized hands-on time makes it Ideal for on-site testing.

Various samples including whole blood can be used.

Rapi:Direct™ Lysis Buffer is a single tube DNA extraction system for rapid preparation of DNA from the variety of samples like cell, tissue, blood and serum which enables direct PCR. The formulation of Rapi:Direct™ Lysis Buffer works in two ways – maximizing the lysis efficiency of biological samples and suppressing inhibitory activities of crude lysates, which maintains the integrity of released genomic DNA. With its minimized hands-on time and good yields, Rapi:Direct™ Lysis Buffer is ideal for on-site PCR testing applications.

RESULTS OF PERFORMANCE VERIFICATION TESTS

[Result of whole blood direct PCR using Rapi:Direct™ Lysis Buffer]

PCR template volume

Lane M100bp DNA marker.

In this test, 10μl of whole blood sample was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 1μl through 5μl of supernatant from the mixture were used as PCR template.

[Result of animal tissue direct PCR using Rapi:Direct™ Lysis Buffer]

PCR template volume

Lane M100bp DNA marker.

In this test, 10mg of porcine tissue was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 2μl through 5μl of supernatant from the mixture were used as PCR template.

PROTOCOL

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ORDERING INFORMATION

Cat. No.
Description
Pack Size
9799140104
Rapi:Direct™ Lysis Buffer 10x
30 mL

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Rapi:Prep™ gDNA Extraction Kit

Simple and Efficient Way to Isolate Genomic DNA

FEATURES

Fast and easy process using spin column makes whole extraction process 20 minutes only.

Uses Silica membrane based spin column for efficient extraction.

No phenol and chloroform is included.

Isolates high quality genomic DNA enabling high yield of PCR amplification.

01 Good extraction process makes your test more reliable.

  • DNA extraction is another important phase to obtain a good result of tests. Genesystem is offering gDNA extraction kit which is well matching with the testing protocols using its ultra-fast PCR platforms.

02 Ideal for fast testing protocol

  • The Rapi:Prep™ gDNA Extraction Kit provides a fast and simple method to isolate genomic DNA from various animal tissues, cultured cells and bacteria. This kit applies silica-membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. The kit is ready for use and can purify the genomic DNA from a wide variety of animal cell and tissue samples, and the whole process is completed in 20 minutes.

EXTRACTION PERFORMANCE COMPARISON

1kb DNA ladder
Kit from Supplier A
Kit from Supplier B
Kit from Supplier C
Kit from Supplier D

Test set-up : Genomic DNA was purified from 20mg of animal tissue using Rapi:Prep™ gDNA Extraction Kit and extraction kits from 4 different competitors respectively. 7μl of the eluate (elution volume: 50 μl) were analyzed on a 1.5% agarose gel.

PROTOCOL

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SPECIFICATION

Format
Silica-membrane based spin column
Sample materials
25mg tissue, 102~106 cultured cells
Typical yield
10~35 μg (depending on sample)
Elution volume
50~100 μl
Duration of process
20 minutes(this can depend on the sample.)

ORDERING INFORMATION

Cat. No.
Description
Pack Size
9799140200
Rapi:Prep™ gDNA Extraction Kit
100 prep

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Media:Direct™ Extraction Buffer

Dramatically reduce extraction time.

FEATURES

Fast and simple method to extract DNA – Whole process in 5 minutes.

Minimized hands-on time.

Ideal for on-site testing.

Room temperature storage.

01 Media:Direct™ Extraction Buffer

  • Media:Direct™ Extraction Buffer is designed to perform PCR test with transport media containing viral samples, without prior DNA/RNA purification process. The buffer includes high concentrated alkaline compounds for lysis and elution of sample organisms. This buffer is produced on the basis of the formulation that minimizes PCR inhibiting contents of the samples. This product is easy to use by just mixing the extraction buffer and transport media without additional process for preparing a nucleic acid template to run PCR tests.

02 RESULTS OF PERFORMANCE VERIFICATION TESTS

  • To comparative experiments between Media:Direct™ Extraction Buffer and others, carried out using GAPDH gene extracted from sample collected by nasopharyngeal and oropharyngeal swab. Each sample was extracted five times per each method. Perform real-time PCR analysis of the extracted nucleic acid from each extraction method using GENECHECKER® UF-300 Real-time PCR System. The relatively lower Ct value was identified than the other methods, which resulted in higher efficiency.

    Media:Direct™ Extraction Buffer

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab(VTM) 30.69 30.83 30.62 30.96 30.31 30.68
    2 Oropharyngeal swab(VTM) 31.58 31.03 31.61 30.08 30.68 31.00

    Spin Column Method ("A" company)

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab(VTM) 31.01 31.12 31.05 30.83 31.07 31.01
    2 Oropharyngeal swab(VTM) 31.51 31.32 31.55 31.53 31.74 31.53

    Magnetic Bead Method ("B" company)

    No. Sample type 1 2 3 4 5 Average
    1 Nasopharyngeal swab(VTM) 34.39 34.34 34.14 34.03 34.58 34.30
    2 Oropharyngeal swab(VTM) 35.23 35.18 34.98 34.55 33.67 34.72

PROTOCOL

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KIT CONTENTS

Item Quantity / Volume Description
Media:Direct™ Extraction Buffer 10 μL x 10 tubes Extraction Buffer

ORDERING INFORMATION

Cat. No.
Description
Pack Size
9799140106
Media:Direct™ Extraction Buffer
10 tubes/PK

You can download further information from below section.

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  • Media:Direct™ Extraction Buffer
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  • · Swab:Direct™ Extraction Kit
  • · Media:Direct™ Extraction Buffer
  • · Rapi:Direct™ Lysis Buffer
  • · Rapi:Prep™ gDNA Extraction Kit